Single cell lineage analysis of mouse embryonic stem cells at the exit from pluripotency.

ABSTRACT: Understanding how interactions between extracellular signalling pathways and transcription factor networks influence cellular decision making will be crucial for understanding mammalian embryogenesis and for generating specialised cell types in vitro. To this end, pluripotent mouse Embryonic Stem (mES) cells have proven to be a useful model system. However, understanding how transcription factors and signalling pathways affect decisions made by individual cells is confounded by the fact that measurements are generally made on groups of cells, whilst individual mES cells differentiate at different rates and towards different lineages, even in conditions that favour a particular lineage. Here we have used single-cell measurements of transcription factor expression and Wnt/β-catenin signallin

SUBMITTER: Trott J 

PROVIDER: S-EPMC3798188 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature