TGF-beta1 does not induce senescence of multipotent mesenchymal stromal cells and has similar effects in early and late passages.

ABSTRACT: Transforming growth factor-beta 1 (TGF-β1) stimulates a broad range of effects which are cell type dependent, and it has been suggested to induce cellular senescence. On the other hand, long-term culture of multipotent mesenchymal stromal cells (MSCs) has a major impact on their cellular physiology and therefore it is well conceivable that the molecular events triggered by TGF-β1 differ considerably in cells of early and late passages. In this study, we analyzed the effect of TGF-β1 on and during replicative senescence of MSCs. Stimulation with TGF-β1 enhanced proliferation, induced a network like growth pattern and impaired adipogenic and osteogenic differentiation. TGF-β1 did not induce premature senescence. However, due to increased proliferation rates the cells reached replicative sene

SUBMITTER: Walenda G 

PROVIDER: S-EPMC3798389 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature