Identification of seroreactive proteins of Leptospira interrogans serovar copenhageni using a high-density protein microarray approach.

ABSTRACT:

Background

Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease.

Methodology

In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line

SUBMITTER: Lessa-Aquino C 

PROVIDER: S-EPMC3798601 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature