XPD-dependent activation of apoptosis in response to triplex-induced DNA damage.

ABSTRACT: DNA sequences capable of forming triplexes are prevalent in the human genome and have been found to be intrinsically mutagenic. Consequently, a balance between DNA repair and apoptosis is critical to counteract their effect on genomic integrity. Using triplex-forming oligonucleotides to synthetically create altered helical distortions, we have determined that pro-apoptotic pathways are activated by the formation of triplex structures. Moreover, the TFIIH factor, XPD, occupies a central role in triggering apoptosis in response to triplex-induced DNA strand breaks. Here, we show that triplexes are capable of inducing XPD-independent double strand breaks, which result in the formation of γH2AX foci. XPD was subsequently recruited to the triplex-induced double strand breaks and co-localized wi

SUBMITTER: Kaushik Tiwari M 

PROVIDER: S-EPMC3799437 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature