In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites.

ABSTRACT: Gene-editing nucleases enable targeted modification of DNA sequences in living cells, thereby facilitating efficient knockout and precise editing of endogenous loci. Engineered nucleases also have the potential to introduce mutations at off-target sites of action. Such unintended alterations can confound interpretation of experiments and can have implications for development of therapeutic applications. Recently, two improved methods for identifying the off-target effects of zinc finger nucleases (ZFNs) were described-one using an in vitro cleavage site selection method and the other exploiting the insertion of integration-defective lentiviruses into nuclease-induced double-stranded DNA breaks. However, application of these two methods to a ZFN pair targeted to the human CCR5 gene led to i

SUBMITTER: Sander JD 

PROVIDER: S-EPMC3799455 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature