The effects of protein solubility on the RNA Integrity Number (RIN) for recombinant Escherichia coli.

ABSTRACT: High quality, intact messenger RNA (mRNA) is required for DNA microarray and reverse transcriptase polymerase chain reaction analysis and is generally obtained from total RNA isolations. The most widely recognized measure of RNA integrity is the RNA Integrity Number (RIN) obtained from the Agilent Bioanalyzer, as it provides sizing, quantification, and quality control measures. This work describes comparisons of the RIN values obtained for recombinant E. coli. Uninduced recombinant E. coli cultures were examined, as well as induced cultures that produced either a soluble or insoluble recombinant protein. The uninduced cultures and the induced cultures producing soluble protein had higher RIN values than the induced cultures producing insoluble protein. These lower RIN values

SUBMITTER: Salazar MA 

PROVIDER: S-EPMC3799817 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature