Comparison of extravillous trophoblast cells derived from human embryonic stem cells and from first trimester human placentas.

ABSTRACT:

Introduction

Preeclampsia and other placental pathologies are characterized by a lack of spiral artery remodeling associated with insufficient invasion by extravillous trophoblast cells (EVT). Because trophoblast invasion occurs in early pregnancy when access to human placental tissue is limited, there is a need for model systems for the study of trophoblast differentiation and invasion. Human embryonic stem cells (hESC) treated with BMP4- differentiate to trophoblast, and express HLA-G, a marker of EVT. The goals of the present study were to further characterize the HLA-G(+) cells derived from BMP4-treated hESC, and determine their suitability as a model.

Methods

HESC were treated with BMP4 under 4% or 20% oxygen and tested in Matrigel invasion chambers. Both BMP4-treated h

SUBMITTER: Telugu BP 

PROVIDER: S-EPMC3799850 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature