Switchable reporter enzymes based on mutually exclusive domain interactions allow antibody detection directly in solution.

ABSTRACT: Detection of antibodies is essential for the diagnosis of many diseases including infections, allergies, and autoimmune diseases. Current heterogeneous immunoassays require multiple time-consuming binding and washing steps, which limits their application in point-of-care diagnostics and high-throughput screening. Here, we report switchable reporter enzymes that allow simple colorimetric detection of antibodies directly in solution. Our approach is based on the antibody-induced disruption of an intramolecular interaction between TEM1 β-lactamase and its inhibitor protein BLIP. Using the HIV1-p17 antibody as an initial target, the interaction between enzyme and inhibitor was carefully tuned to yield a reporter enzyme whose activity increased 10-fold in the presence of pM antibody concentrati

SUBMITTER: Banala S 

PROVIDER: S-EPMC3800467 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature