Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation.

ABSTRACT:

Purpose

Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation.

Methods

Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed b

SUBMITTER: Intasqui P 

PROVIDER: S-EPMC3800528 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature