Maternal obesity enhances white adipose tissue differentiation and alters genome-scale DNA methylation in male rat offspring.

ABSTRACT: The risk of obesity (OB) in adulthood is strongly influenced by maternal body composition. Here we examined the hypothesis that maternal OB influences white adipose tissue (WAT) transcriptome and increases propensity for adipogenesis in the offspring, prior to the development of OB, using an established model of long-term metabolic programming. Employing an overfeeding-based rat model, in which exposure to OB is limited to preconception and gestation alone, we conducted global transcriptomic profiling in WAT, and gene/protein expression analysis of lipogenic and adipogenic pathways and examined adipogenic differentiation of WAT stromal-vascular cells ex vivo. Using reduced representation bisulfite sequencing we also evaluated genome-scale changes in DNA methylation in offspring WAT. Matern

SUBMITTER: Borengasser SJ 

PROVIDER: S-EPMC3800750 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature