Atomic force microscopy captures folded ribosome bound nascent chains.

ABSTRACT: Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic "horse-shoe" shape of ankyrin-R emerging from the ribosome was captured.

SUBMITTER: Loksztejn A 

PROVIDER: S-EPMC3804001 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature