Industrial robustness: understanding the mechanism of tolerance for the Populus hydrolysate-tolerant mutant strain of Clostridium thermocellum.

ABSTRACT:

Background

An industrially robust microorganism that can efficiently degrade and convert lignocellulosic biomass into ethanol and next-generation fuels is required to economically produce future sustainable liquid transportation fuels. The anaerobic, thermophilic, cellulolytic bacterium Clostridium thermocellum is a candidate microorganism for such conversions but it, like many bacteria, is sensitive to potential toxic inhibitors developed in the liquid hydrolysate produced during biomass processing. Microbial processes leading to tolerance of these inhibitory compounds found in the pretreated biomass hydrolysate are likely complex and involve multiple genes.

Methodology/principal findings

In this study, we developed a 17.5% v/v Populus hydrolysate tolerant mutant strain of

SUBMITTER: Linville JL 

PROVIDER: S-EPMC3804516 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature