An open source based high content screening method for cell biology laboratories investigating cell spreading and adhesion.

ABSTRACT:

Background

Adhesion dependent mechanisms are increasingly recognized to be important for a wide range of biological processes, diseases and therapeutics. This has led to a rising demand of pharmaceutical modulators. However, most currently available adhesion assays are time consuming and/or lack sensitivity and reproducibility or depend on specialized and expensive equipment often only available at screening facilities. Thus, rapid and economical high-content screening approaches are urgently needed.

Results

We established a fully open source high-content screening method for identifying modulators of adhesion. We successfully used this method to detect small molecules that are able to influence cell adhesion and cell spreading of Swiss-3T3 fibroblasts in general and/or spec

SUBMITTER: Schmandke A 

PROVIDER: S-EPMC3804740 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature