Expression of recombinant streptokinase from streptococcus pyogenes and its reaction with infected human and murine sera.

ABSTRACT:

Objective(s)

Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analysis.

Materials and methods

In this study, the ska gene from S. pyogenes was amplified and cloned into pET32a which is a prokaryotic expression vector. pET32a-ska was transformed to Escherichia coli BL21 (DE3) pLysS and gene expression was induced by IPTG. Protein production was improved by modification of composition of the bacterial culture media and altering the induction time by IPTG.

SUBMITTER: Molaee N 

PROVIDER: S-EPMC3804847 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature