Bacteriocin from Bacillus subtilis as a novel drug against diabetic foot ulcer bacterial pathogens.

ABSTRACT:

Objective

To isolate and identify Bacillus subtilis (B. subtilis) from soil and to characterize and partially purify the bacteriocin. To evaluate the antimicrobial activity against four diabetic foot ulcer bacterial pathogens.

Methods

Genotypic identification was done based on Bergey's manual of systemic bacteriology. Antimicrobial susceptibility test was done by Kirby-Bauer disc diffusion method. Colonies were identified by colony morphology and biochemical characterization and also compared with MTCC 121 strain. Further identification was done by 16S rRNA sequencing. Inhibitory activities of partially purified bacteriocin on all the DFU isolates were done by agar well diffusion method. The strain was identified to produce bacteriocin by stab overlay assay. Bacteriocin was

SUBMITTER: Joseph B 

PROVIDER: S-EPMC3805101 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature