Cord-blood engraftment with ex vivo mesenchymal-cell coculture.

ABSTRACT:

Background

Poor engraftment due to low cell doses restricts the usefulness of umbilical-cord-blood transplantation. We hypothesized that engraftment would be improved by transplanting cord blood that was expanded ex vivo with mesenchymal stromal cells.

Methods

We studied engraftment results in 31 adults with hematologic cancers who received transplants of 2 cord-blood units, 1 of which contained cord blood that was expanded ex vivo in cocultures with allogeneic mesenchymal stromal cells. The results in these patients were compared with those in 80 historical controls who received 2 units of unmanipulated cord blood.

Results

Coculture with mesenchymal stromal cells led to an expansion of total nucleated cells by a median factor of 12.2 and of CD34+ cells by a median fa

SUBMITTER: de Lima M 

PROVIDER: S-EPMC3805360 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature