Evaluation and validation of reference genes for normalization of quantitative real-time PCR based gene expression studies in peanut.

ABSTRACT: The quantitative real-time PCR (qPCR) based techniques have become essential for gene expression studies and high-throughput molecular characterization of transgenic events. Normalizing to reference gene in relative quantification make results from qPCR more reliable when compared to absolute quantification, but requires robust reference genes. Since, ideal reference gene should be species specific, no single internal control gene is universal for use as a reference gene across various plant developmental stages and diverse growth conditions. Here, we present validation studies of multiple stably expressed reference genes in cultivated peanut with minimal variations in temporal and spatial expression when subjected to various biotic and abiotic stresses. Stability in the expression of eigh

SUBMITTER: Reddy DS 

PROVIDER: S-EPMC3805511 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature