Comparative transcriptional profiling of 3 murine models of SLE nephritis reveals both unique and shared regulatory networks.

ABSTRACT:

Objective

To define shared and unique features of SLE nephritis in mouse models of proliferative and glomerulosclerotic renal disease.

Methods

Perfused kidneys from NZB/W F1, NZW/BXSB and NZM2410 mice were harvested before and after nephritis onset. Affymetrix based gene expression profiles of kidney RNA were analyzed using Genomatix Pathway Systems and Ingenuity Pathway Analysis software. Gene expression patterns were confirmed using real-time PCR.

Results

955, 1168 and 755 genes were regulated in the kidneys of nephritic NZB/W F1, NZM2410 and NZW/BXSB mice respectively. 263 genes were regulated concordantly in all three strains reflecting immune cell infiltration, endothelial cell activation, complement activation, cytokine signaling, tissue remodeling and hypoxia.

SUBMITTER: Bethunaickan R 

PROVIDER: S-EPMC3805607 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature