High-throughput 3D screening reveals differences in drug sensitivities between culture models of JIMT1 breast cancer cells.

ABSTRACT: The traditional method for studying cancer in vitro is to grow immortalized cancer cells in two-dimensional monolayers on plastic. However, many cellular features are impaired in these artificial conditions, and large changes in gene expression compared to tumors have been reported. Three-dimensional cell culture models have become increasingly popular and are suggested to be better models than two-dimensional monolayers due to improved cell-to-cell contact and structures that resemble in vivo architecture. The aim of this study was to develop a simple high-throughput three-dimensional drug screening method and to compare drug responses in JIMT1 breast cancer cells when grown in two dimensions, in poly(2-hydroxyethyl methacrylate) induced anchorage-independent three-dimensional models, and

SUBMITTER: Hongisto V 

PROVIDER: S-EPMC3806867 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature