Generation of multiple replication-competent retroviruses through recombination between PreXMRV-1 and PreXMRV-2.

ABSTRACT: We previously identified two novel endogenous murine leukemia virus proviruses, PreXMRV-1 and PreXMRV-2, and showed that they most likely recombined during xenograft passaging of a human prostate tumor in mice to generate xenotropic murine leukemia virus-related virus (XMRV). To determine the recombination potential of PreXMRV-1 and PreXMRV-2, we examined the generation of replication-competent retroviruses (RCRs) over time in a cell culture system. We observed that either virus alone was noninfectious and the RNA transcripts of the viruses were undetectable in the blood and spleen of nude mice that carry them. To determine their potential to generate RCRs through recombination, we transfected PreXMRV-1 and PreXMRV-2 into 293T cells and used the virus produced to infect fresh cells; the pr

SUBMITTER: Delviks-Frankenberry K 

PROVIDER: S-EPMC3807343 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature