Exploring the roles of DNA methylation in the metal-reducing bacterium Shewanella oneidensis MR-1.

ABSTRACT: We performed whole-genome analyses of DNA methylation in Shewanella oneidensis MR-1 to examine its possible role in regulating gene expression and other cellular processes. Single-molecule real-time (SMRT) sequencing revealed extensive methylation of adenine (N6mA) throughout the genome. These methylated bases were located in five sequence motifs, including three novel targets for type I restriction/modification enzymes. The sequence motifs targeted by putative methyltranferases were determined via SMRT sequencing of gene knockout mutants. In addition, we found that S. oneidensis MR-1 cultures grown under various culture conditions displayed different DNA methylation patterns. However, the small number of differentially methylated sites could not be directly linked to the much larger numbe

SUBMITTER: Bendall ML 

PROVIDER: S-EPMC3807482 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature