Gene expression responses to mechanical stimulation of mesenchymal stem cells seeded on calcium phosphate cement.

ABSTRACT:

Introduction

The aim of the study reported here was to investigate the molecular responses of human mesenchymal stem cells (MSC) to loading with a model that attempts to closely mimic the physiological mechanical loading of bone, using monetite calcium phosphate (CaP) scaffolds to mimic the biomechanical properties of bone and a bioreactor to induce appropriate load and strain.

Methods

Human MSCs were seeded onto CaP scaffolds and subjected to a pulsating compressive force of 5.5±4.5 N at a frequency of 0.1 Hz. Early molecular responses to mechanical loading were assessed by microarray and quantitative reverse transcription-polymerase chain reaction and activation of signal transduction cascades was evaluated by western blotting analysis.

Results

The maximum mechanica

SUBMITTER: Gharibi B 

PROVIDER: S-EPMC3807700 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature