Circulating microRNA is a biomarker of pediatric Crohn disease.

ABSTRACT:

Objective

The gold standard for the diagnosis and evaluation of Crohn disease (CD) is endoscopy/colonoscopy, although this is invasive, costly, and associated with risks to the patient. Recently, circulating microRNAs (miRNAs) have emerged as promising noninvasive biomarkers. Here, we examined the utility of serum miRNAs as biomarkers of CD in children.

Patients and methods

Studies were conducted using sera samples from patients with pediatric CD, healthy controls, and a comparison group of patients with pediatric celiac disease. Serum miRNA levels were explored initially using a microfluidic quantitative reverse transcription-polymerase chain reaction array platform. Findings were subsequently validated using quantitative reverse transcription-polymerase chain reaction in l

SUBMITTER: Zahm AM 

PROVIDER: S-EPMC3807879 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature