Epstein-Barr virus induces global changes in cellular mRNA isoform usage that are important for the maintenance of latency.

ABSTRACT: Oncogenic viruses promote cell proliferation through the dramatic reorganization of host transcriptomes. In addition to regulating mRNA abundance, changes in mRNA isoform usage can have a profound impact on the protein output of the transcriptome. Using Epstein-Barr virus (EBV) transformation of primary B cells, we have studied the ability of an oncogenic virus to alter the mRNA isoform profile of its host. Using the algorithm called SplicerEX with two complementary Affymetrix microarray platforms, we uncovered 433 mRNA isoform changes regulated by EBV during B-cell transformation. These changes were largely orthogonal with the 2,163 mRNA abundance changes observed during transformation, such that less than one-third of mRNAs changing at the level of isoform also changed in overall abundan

SUBMITTER: Homa NJ 

PROVIDER: S-EPMC3807935 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature