Analysis of amide compounds in different parts of Piper ovatum Vahl by high-performance liquid chromatographic.

ABSTRACT:

Background

Piper ovatum (Piperaceae) has been used in traditional medicine for the treatment of inflammations and as an analgesic. Previous studies have showed important biological activities of the extracts and amides from P. ovatum leaves.

Objective

In this study, a high-performance liquid chromatographic (HPLC) method was developed and validated for quantitative determination of the amides in different parts of Piper ovatum.

Materials and methods

The analysis was carried out on a Metasil ODS column (150 × 4.6 mm, 5μm) at room temperature. HPLC conditions were as follows: acetonitrile (A), and water (B), 1.0% acetic acid. The gradient elution used was 0-30 min, 0-60% A; 30-40 min, 60% A. Flow rate used was 1.0mL/min, and detection at 280nm.

Results

The valida

SUBMITTER: Silva DR 

PROVIDER: S-EPMC3807989 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature