Variations in both TG1 and TG2 isozyme-specific in situ activities and protein expressions during mouse embryonic development.

ABSTRACT: Transglutaminase (TG) is a family of enzymes that catalyzes cross-linking reactions among proteins. Using fluorescent-labeled highly reactive substrate peptides, we recently developed a system to visualize isozyme-specific in situ enzymatic activity. In the present study, we investigated the in situ activities of TG1 (skin-type) and TG2 (tissue-type) using whole mouse sections of various embryonic developmental stages and neonates. In each case, we also successfully used immunostaining of identical whole mouse sections for protein expression after detection of enzymatic activities. In general, the enzymatic activity was correlated with TG protein expression. However, in some tissues, TG protein expression patterns, which were inconsistent with the enzymatic activities, suggested that inact

SUBMITTER: Itoh M 

PROVIDER: S-EPMC3808576 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature