Application of systems biology principles to protein biomarker discovery: urinary exosomal proteome in renal transplantation.

ABSTRACT:

Purpose

In mass spectrometry (MS)-based studies to discover urinary protein biomarkers, an important question is how to analyze the data to find the most promising potential biomarkers to be advanced to large-scale validation studies. Here, we describe a "systems biology-based" approach to address this question.

Experimental design

We analyzed large-scale liquid chromatography-tandem mass spectrometry (LC-MS/MS) data of urinary exosomes from renal allograft recipients with biopsy-proven evidence of immunological rejection or tubular injury (TI). We asked whether bioinformatic analysis of urinary exosomal proteins can identify biological-process based protein groups that correlate with biopsy findings and whether the protein groups fit with general knowledge of the pathophysi

SUBMITTER: Pisitkun T 

PROVIDER: S-EPMC3809079 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature