Comparison of interactions of diamine and Mg²⁺ with RNA tertiary structures: similar versus differential effects on the stabilities of diverse RNA folds.

ABSTRACT: Cations play a large role in stabilizing the native state of RNA in vivo. In addition to Mg²⁺, putrescine²⁺ is an abundant divalent cation in bacterial cells, but its effect on the folding of RNA tertiary structure has not been widely explored. In this study, we look at how the stabilities of four structured RNAs, each with a different degree of dependence on K⁺ and Mg²⁺, are affected by putrescine²⁺ relative to Mg²⁺. Through the use of thermal melts, we observe that (i) at a given concentration, putrescine²⁺ is less effective than Mg²⁺ at stabilizing RNA, (ii) the stability imparted to RNA by various diamines is a function of charge density (average separation distance between charges) as well as the flexibility of the counterion, and (iii) when Mg²⁺ is already present in a buffer, furthe

SUBMITTER: Trachman RJ 

PROVIDER: S-EPMC3809084 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature