Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns.

ABSTRACT: Alterations in cell shape have been shown to modulate chromatin condensation and cell lineage specification; however, the mechanisms controlling these processes are largely unknown. Because endothelial cells experience cyclic mechanical changes from blood flow during normal physiological processes and disrupted mechanical changes as a result of abnormal blood flow, cell shape deformation and loss of polarization during coronary artery disease, we aimed to determine how morphological restriction affects global gene expression patterns. Human coronary artery endothelial cells (HCAECs) were cultured on spatially defined adhesive micropatterns, forcing them to conform to unique cellular morphologies differing in cellular polarization and angularity. We utilized pattern recognition algorithms a

SUBMITTER: Stiles JM 

PROVIDER: S-EPMC3810024 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature