SAMstrt: statistical test for differential expression in single-cell transcriptome with spike-in normalization.

ABSTRACT:

Motivation

Recent transcriptome studies have revealed that total transcript numbers vary by cell type and condition; therefore, the statistical assumptions for single-cell transcriptome studies must be revisited. SAMstrt is an extension code for SAMseq, which is a statistical method for differential expression, to enable spike-in normalization and statistical testing based on the estimated absolute number of transcripts per cell for single-cell RNA-seq methods.

Availability and implementation

SAMstrt is implemented on R and available in github (https://github.com/shka/R-SAMstrt).

Contact

[e-mail]

SUBMITTER: Katayama S 

PROVIDER: S-EPMC3810855 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature