Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance.

ABSTRACT: With increasing numbers of hospital-acquired antibiotic resistant infections each year and staggering health care costs, there is a clear need for new antimicrobial agents, as well as novel strategies to extend their clinical efficacy. While genomic studies have provided a wealth of information about the alleles associated with adaptation to antibiotics, they do not provide essential information about the relative importance of genomic changes, their order of appearance, or potential epistatic relationships between adaptive changes. Here we used quantitative experimental evolution of a single polymorphic population in continuous culture with whole-genome sequencing and allelic frequency measurements to study daptomycin (DAP) resistance in the vancomycin-resistant clinical pathogen Enteroco

SUBMITTER: Miller C 

PROVIDER: S-EPMC3811304 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature