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Clostridium acidurici electron-bifurcating formate dehydrogenase.

ABSTRACT: Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.

SUBMITTER: Wang S 

PROVIDER: S-EPMC3811350 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Clostridium acidurici electron-bifurcating formate dehydrogenase.

Wang Shuning S   Huang Haiyan H   Kahnt Jörg J   Thauer Rudolf K RK  

Applied and environmental microbiology 20130719 19

Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2. ...[more]

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