SbcCD-mediated processing of covalent gyrase-DNA complex in Escherichia coli.

ABSTRACT: Quinolones trap the covalent gyrase-DNA complex in Escherichia coli, leading to cell death. Processing activities for trapped covalent complex have not been characterized. A mutant strain lacking SbcCD nuclease activity was examined for both accumulation of gyrase-DNA complex and viability after quinolone treatment. Higher complex levels were found in ΔsbcCD cells than in wild-type cells after incubation with nalidixic acid and ciprofloxacin. However, SbcCD activity protected cells against the bactericidal action of nalidixic acid but not ciprofloxacin.

SUBMITTER: Aedo S 

PROVIDER: S-EPMC3811449 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature