Novel determinants of intestinal colonization of Salmonella enterica serotype typhimurium identified in bovine enteric infection.

ABSTRACT: Cattle are naturally infected with Salmonella enterica serotype Typhimurium and exhibit pathological features of enteric salmonellosis that closely resemble those in humans. Cattle are the most relevant model of gastrointestinal disease resulting from nontyphoidal Salmonella infection in an animal with an intact microbiota. We utilized this model to screen a library of targeted single-gene deletion mutants to identify novel genes of Salmonella Typhimurium required for survival during enteric infection. Fifty-four candidate mutants were strongly selected, including numerous mutations in genes known to be important for gastrointestinal survival of salmonellae. Three genes with previously unproven phenotypes in gastrointestinal infection were tested in bovine ligated ileal loops. Two of these

SUBMITTER: Elfenbein JR 

PROVIDER: S-EPMC3811824 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature