A new short oligonucleotide-based strategy for the precursor-specific regulation of microRNA processing by dicer.

ABSTRACT: The precise regulation of microRNA (miRNA) biogenesis seems to be critically important for the proper functioning of all eukaryotic organisms. Even small changes in the levels of specific miRNAs can initiate pathological processes, including carcinogenesis. Accordingly, there is a great need to develop effective methods for the regulation of miRNA biogenesis and activity. In this study, we focused on the final step of miRNA biogenesis; i.e., miRNA processing by Dicer. To test our hypothesis that RNA molecules can function not only as Dicer substrates but also as Dicer regulators, we previously identified by SELEX a pool of RNA oligomers that bind to human Dicer. We found that certain of these RNA oligomers could selectively inhibit the formation of specific miRNAs. Here, we show that these

SUBMITTER: Kurzynska-Kokorniak A 

PROVIDER: S-EPMC3812226 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature