Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene.

ABSTRACT:

Aim

To investigate reprogramming of human adipose tissue derived stem cells into insulin producing cells using non-integrated lentivirus harboring PDX1 gene.

Methods

In this study, human adipose tissue derived stem cells (hADSCs) were obtained from abdominal adipose tissues by liposuction, selected by plastic adhesion, and characterized by flow cytometric analysis. Human ADSCs were differentiated into adipocytes and osteocytes using differentiating medium to confirm their multipotency. Non-integrated lentiviruses harboring PDX1 (Non-integrated LV-PDX1) were constructed using specific plasmids (pLV-HELP, pMD2G, LV-105-PDX1-1). Then, hADSCs were transduced with non-integrated LV-PDX1. After transduction, ADSCs(PDX1+) were cultured in high glucose DMEM medium supplement by B27,

SUBMITTER: Boroujeni ZN 

PROVIDER: S-EPMC3812525 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature