Microbiota separation and C-reactive protein elevation in treatment-naive pediatric granulomatous Crohn disease.

ABSTRACT:

Objectives

In patients with inflammatory bowel diseases (IBDs), the presence of noncaseating mucosal granuloma is sufficient for diagnosing Crohn disease (CD) and may represent a specific immune response or microbial-host interaction. The cause of granulomas in CD is unknown and their association with the intestinal microbiota has not been addressed with high-throughput methodologies.

Methods

The mucosal microbiota from 3 different pediatric centers was studied with 454 pyrosequencing of the bacterial 16S rRNA gene and the fungal small subunit (SSU) ribosomal region in transverse colonic biopsy specimens from 26 controls and 15 treatment-naïve pediatric CD cases. Mycobacterium avium subspecies paratuberculosis (MAP) was tested with real-time polymerase chain reaction. The co

SUBMITTER: Kellermayer R 

PROVIDER: S-EPMC3812911 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature