Gene gymnastics: Synthetic biology for baculovirus expression vector system engineering.

ABSTRACT: Most essential activities in eukaryotic cells are catalyzed by large multiprotein assemblies containing up to ten or more interlocking subunits. The vast majority of these protein complexes are not easily accessible for high resolution studies aimed at unlocking their mechanisms, due to their low cellular abundance and high heterogeneity. Recombinant overproduction can resolve this bottleneck and baculovirus expression vector systems (BEVS) have emerged as particularly powerful tools for the provision of eukaryotic multiprotein complexes in high quality and quantity. Recently, synthetic biology approaches have begun to make their mark in improving existing BEVS reagents by de novo design of streamlined transfer plasmids and by engineering the baculovirus genome. Here we present OmniBac, co

SUBMITTER: Vijayachandran LS 

PROVIDER: S-EPMC3813527 | biostudies-literature | 2013 Sep-Oct

REPOSITORIES: biostudies-literature