CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.

ABSTRACT: The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.

SUBMITTER: Waaijers S 

PROVIDER: S-EPMC3813849 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature