New levels of transcriptome complexity at upper thermal limits in wild Drosophila revealed by exon expression analysis.

ABSTRACT: While the cellular heat-shock response has been a paradigm for studying the impact of thermal stress on RNA metabolism and gene expression, the genome-wide response to thermal stress and its connection to physiological stress resistance remain largely unexplored. Here, we address this issue using an array-based exon expression analysis to interrogate the transcriptome in recently established Drosophila melanogaster stocks during severe thermal stress and recovery. We first demonstrated the efficacy of exon-level analyses to reveal a level of thermally induced transcriptome complexity extending well beyond gene-level analyses. Next, we showed that the upper range of both the cellular and physiological thermal stress response profoundly affected message expression and processing in D. melano

SUBMITTER: Telonis-Scott M 

PROVIDER: S-EPMC3813866 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature