How changes in affinity of corticosteroid-binding globulin modulate free cortisol concentration.

ABSTRACT:

Context

Recent studies of corticosteroid-binding globulin (CBG) indicate that it does not merely transport cortisol passively but also actively regulates its release in the circulation. We show how CBG binding affinity can vary to give changes in free cortisol concentration in a physiologically relevant range.

Objective

The objective was to determine how the binding affinity of plasma CBG is affected by glycosylation, changes in body temperature, and the conformational change induced by proteases at sites of inflammation.

Design

Binding assays were performed over a range of temperatures with plasma and recombinant CBG to determine the contribution of glycosylation. The role of conformational change was assessed by measuring binding affinities of plasma CBG before and

SUBMITTER: Chan WL 

PROVIDER: S-EPMC3813945 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature