Genome-wide single-cell-level screen for protein abundance and localization changes in response to DNA damage in S. cerevisiae.

ABSTRACT: An effective response to DNA damaging agents involves modulating numerous facets of cellular homeostasis in addition to DNA repair and cell-cycle checkpoint pathways. Fluorescence microscopy-based imaging offers the opportunity to simultaneously interrogate changes in both protein level and subcellular localization in response to DNA damaging agents at the single-cell level. We report here results from screening the yeast Green Fluorescent Protein (GFP)-fusion library to investigate global cellular protein reorganization on exposure to the alkylating agent methyl methanesulfonate (MMS). Broad groups of induced, repressed, nucleus- and cytoplasm-enriched proteins were identified. Gene Ontology and interactome analyses revealed the underlying cellular processes. Transcription factor (TF) ana

SUBMITTER: Mazumder A 

PROVIDER: S-EPMC3814357 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature