Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease.

ABSTRACT: The generation of genome-modified animals is a powerful approach to analyze gene functions. The CAS9/guide RNA (gRNA) system is expected to become widely used for the efficient generation of genome-modified animals, but detailed studies on optimum conditions and availability are limited. In the present study, we attempted to generate large-scale genome-modified mice with an optimized CAS9/gRNA system, and confirmed the transmission of these mutations to the next generations. A comparison of different types of gRNA indicated that the target loci of almost all pups were modified successfully by the use of long-type gRNAs with CAS9. We showed that this system has much higher mutation efficiency and much lower off-target effect compared to zinc-finger nuclease. We propose that most of these of

SUBMITTER: Fujii W 

PROVIDER: S-EPMC3814358 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature