In planta protein interactions of three alphacryptoviruses and three betacryptoviruses from White Clover, Red Clover and Dill by bimolecular fluorescence complementation analysis.

ABSTRACT: Plant-infecting viruses of the genera Alpha- and Betacryptovirus within the family Partitiviridae cause no visible effects on their hosts and are only transmitted by cell division and through gametes. The bipartite dsRNA genome is encoding a RNA-dependent RNA polymerase (RdRp) and a coat protein (CP). Aside from sequence and structural analysis, the investigation of protein interactions is another step towards virus characterization. Therefore, ORFs of two type members White Clover Cryptic Virus 1 and 2 (WCCV-1 and WCCV-2), as well as the related viruses from Red Clover and Dill were introduced into a bimolecular fluorescence complementation assay. We showed CP-CP dimerization for all tested viruses with localization for alphacryptoviruses at the nuclear membrane and for betacryptoviruses

SUBMITTER: Lesker T 

PROVIDER: S-EPMC3814600 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature