Comparative evaluation of miRNA expression between in vitro and in vivo airway epithelium demonstrates widespread differences.

ABSTRACT: Airway epithelial cells cultured at an air-liquid interface bear many hallmarks of in vivo cells and are used extensively to study the biology of the lung epithelium. Because miRNAs regulate many cellular functions, we postulated that miRNA profiling would provide an unbiased assessment of the effects of in vitro culturing. RNA was extracted from primary airway epithelial cells either immediately after cell procurement (in vivo condition) or after air-liquid interface culture was established (in vitro condition). We assessed 742 miRNAs and determined differential expression between in vivo and in vitro conditions. Air-liquid interface culturing of airway epithelial cells caused widespread changes in miRNA expression. A similarly extensive alteration in gene expression was observed in an in

SUBMITTER: Chen P 

PROVIDER: S-EPMC3814685 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature