Study of the HIV-2 Env cytoplasmic tail variability and its impact on Tat, Rev and Nef.

ABSTRACT:

Background

The HIV-2 env's 3' end encodes the cytoplasmic tail (CT) of the Env protein. This genomic region also encodes the rev, Tat and Nef protein in overlapping reading frames. We studied the variability in the CT coding region in 46 clinical specimens and in 2 reference strains by sequencing and by culturing. The aims were to analyse the variability of Env CT and the evolution of proteins expressed from overlapping coding sequences.

Results

A 70% reduction of the length of the CT region affected the HIV-2 ROD and EHO strains in vitro due to a premature stop codon in the env gene. In clinical samples this wasn't observed, but the CT length varied due to insertions and deletions. We noted 3 conserved and 3 variable regions in the CT. The conserved regions were those conta

SUBMITTER: Bakouche N 

PROVIDER: S-EPMC3815105 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature