Dynamic interplay between the periplasmic and transmembrane domains of GspL and GspM in the type II secretion system.

ABSTRACT: The type II secretion system (T2SS) is a multiprotein nanomachine that transports folded proteins across the outer membrane of gram-negative bacteria. The molecular mechanisms that govern the secretion process remain poorly understood. The inner membrane components GspC, GspL and GspM possess a single transmembrane segment (TMS) and a large periplasmic region and they are thought to form a platform of unknown function. Here, using two-hybrid and pull-down assays we performed a systematic mapping of the GspC/GspL/GspM interaction regions in the plant pathogen Dickeya dadantii. We found that the TMS of these components interact with each other, implying a complex interaction network within the inner membrane. We also showed that the periplasmic, ferredoxin-like, domains of GspL and GspM driv

SUBMITTER: Lallemand M 

PROVIDER: S-EPMC3815138 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature