An optimized whole-body cortisol quantification method for assessing stress levels in larval zebrafish.

ABSTRACT: Glucocorticoids serve important regulatory functions for many physiological processes and are critical mediators of the stress response. The stress response is a set of bodily processes aimed at counteracting a state of threatened homeostasis. Proper stress response is critical for the survival of an animal, however prolonged or abnormal stress response can be detrimental and is implicated in a number of human diseases such as depression and metabolic diseases. To dissect the underlying mechanism of this complex and important response, the zebrafish, Danio rerio offer important advantages such as ease of genetic manipulations and high-throughput behavioral analyses. However, there is a paucity of suitable methods to measure stress level in larval zebrafish. Therefore, an efficient low-cost

SUBMITTER: Yeh CM 

PROVIDER: S-EPMC3815139 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature