Constructing and testing the thermodynamic limits of synthetic NAD(P)H:H2 pathways.

ABSTRACT: NAD(P)H:H(2) pathways are theoretically predicted to reach equilibrium at very low partial headspace H(2) pressure. An evaluation of the directionality of such near-equilibrium pathways in vivo, using a defined experimental system, is therefore important in order to determine its potential for application. Many anaerobic microorganisms have evolved NAD(P)H:H(2) pathways; however, they are either not genetically tractable, and/or contain multiple H(2) synthesis/consumption pathways linked with other more thermodynamically favourable substrates, such as pyruvate. We therefore constructed a synthetic ferredoxin-dependent NAD(P)H:H(2) pathway model system in Escherichia coli BL21(DE3) and experimentally evaluated the thermodynamic limitations of nucleotide pyridine-dependent H(2) synthesis und

SUBMITTER: Veit A 

PROVIDER: S-EPMC3815245 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature