Use of mycelia as paths for the isolation of contaminant-degrading bacteria from soil.

ABSTRACT: Mycelia of fungi and soil oomycetes have recently been found to act as effective paths boosting bacterial mobility and bioaccessibility of contaminants in vadose environments. In this study, we demonstrate that mycelia can be used for targeted separation and isolation of contaminant-degrading bacteria from soil. In a 'proof of concept' study we developed a novel approach to isolate bacteria from contaminated soil using mycelia of the soil oomycete Pythium ultimum as translocation networks for bacteria and the polycyclic aromatic hydrocarbon naphthalene (NAPH) as selective carbon source. NAPH-degrading bacterial isolates were affiliated with the genera Xanthomonas, Rhodococcus and Pseudomonas. Except for Rhodococcus the NAPH-degrading isolates exhibited significant motility as observed in s

SUBMITTER: Furuno S 

PROVIDER: S-EPMC3815281 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature